各种激发光和吸收光波长.docx

1、各种激发光和吸收光波长各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染色时是必不可少的参考资料。全部文件请到这里下载！Excitation and Emission Wavelengths of FluorophoresTavis FluoroTable: Common fluorophores(from Zeiss Corporation website)FluorophoreAbsorptionEmissionOther info1,5 IAEDANS 3364901,8-ANS3724804-Methylumbelliferone385

2、5025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (HydroxyTryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX

3、 (carboxy-X-rhodamine)578567604591 5-TAMRA (5-Carboxytetramethylrhodamine)548542552568 6-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Ac

4、id Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520AcriflavinFeulgen SITSA355-425460Aequorin (Photoprotein)466Photop

5、roteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350346342442441 Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556, 557572, 573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 63363265

6、0Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560, 580580, 624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345347425444 AMCA-X353442Aminoactinomycin D555655Aminocoumarin346350442445 Aminomethylcoumar

7、in (AMCA)345347425444 Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490

8、ATTO-TAG? CBQCA465560ATTO-TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520BerberineSulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445,

9、 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV370435BOBO?-1462481BOBO?-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/5105

10、09515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676BodipyFl504, 505511, 513Bodipy FL ATP505514BodipyFl-Ceramide505511Bodipy R6G SE528547Bodipy

11、 TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO?-1462481BO-PRO?-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue

12、373440Calcium Crimson588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye 506531Ca2+ DyeCalcium Green-2 Ca2+ 506/503536Ca2+Calcium Green-5N Ca2+ 506532Ca2+Calcium Green-C18 Ca2+ 509530Ca2+Calcium Orange549575576 Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX)576601C

13、ascade Blue377398399420423 Cascade Yellow399400550552 Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRETChlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ra

14、tio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence, native moleculeCoelenterazinecp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceCoelenterazine f(437)473Ca2+ Dye, bioluminescenceCoelenterazinefcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye,

15、 bioluminescenceCoelenterazinehcp(433)444Ca2+ Dye, bioluminescenceCoelenterazineip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575CoumarinPhalloidin387470C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 855

16、4568Cy3.5581598Cy3514552554566570 Cy5.1 8649666Cy5.5675695Cy5649666670 Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell Proliferation AssayDabcyl453Dansyl340578Dansyl Amine337517DansylCadaverine335518Dansyl Chloride372518Da

17、nsyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488498605713 DiA (4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetat

18、e (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501, 502DiR748780Lipophilic TracerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-5

19、20DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enhanced Blue Fluorescent ProteinECFP436474Enhanced Cyan Fluorescent ProteinEGFP488, 498507, 516Enhanced Green Fluorescent ProteinELF 97345530

20、Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidiumhomodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced Yellow Fluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Ind

21、uced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 ?506, 506520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fu

22、ra Red?(high pH)572657Fura Red?Fluo-3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifte

23、d (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347Filter sets installed on the Axiovert 200M:1) Brightfield DIC:filter cube contains DIC

24、analyzer2) DAPI / BFP: Chroma set 31041, Ex at 380 nm (bandwidth 30 nm), Em at 460 nm (bandwidth 50 nm).3) FITC / GFP:Chroma set 41001, Ex at 480 nm (bandwidth 40 nm), Em at 535 nm (band width 50 nm).4) RHOD:Chroma set 41002c, Ex at 545 nm (bandwidth 30 nm), Em at 620 nm (bandwidth 60 nm). 5) Cy5:Ch

25、roma set 41024, Ex at 620 nm (bandwidth 60 nm), Em 665 nm and above (long pass filter). Also available upon request:4) GFP:Chroma set 41017, Ex at 470 nm (bandwidth 40 nm), Em 525 nm (band width 50 nm).5) CFP:Chroma set 31044v2, Ex at 436 nm (bandwidth 20 nm), Em 480 nm (bandwidth 40 nm).6) YFP: Chroma set 41028, Ex at 500 (bandwidth 20 nm), Em 535 (bandwidth 30 nm).7) CFP/YFP FRET: Chroma set 31052, Ex 436 (bandwidth 20 nm), Em 535 (bandwidth 30 nm).