DNA内切酶名称位点序列及切点便于查找.docx

1、DNA内切酶名称位点序列及切点便于查找DNA内切酶名称、位点序列及切点(便于查找)22. The chemical tool of claim 21 wherein the restriction endonuclease for targeting DNA sequences is selected from the group consisting of:Sequence cutEnzyme NameSEQ ID NO:AAR/AATTYAcsI53R/AATTYApoI53A/AGCTTHindIII54AA/CGTTPsp140 6I55AAT/ATTSspI56ACA/CRYGTAf

2、lIII57A/CCGGTAgeI58A/CATGTBspLU11I59ACCTGC(4,8)BspMI60, 61ACTGG(1,611)BsrI62, 63R/CCGGYBsrFI64R/CCGGYCfr10I64A/CGTMaeII65A/CGCGTMluI66RCATG/YNspI67A/CCGGTPinAI58A/CCWGGTSexAI68A/CTAGTSpeI69AGAGG/CCTAatI70AG/CTAluI71A/GATCTBglII72R/GATCYBstYI73RG/GNCCYDraII74AGC/GCTEco47III75RG/GNCCYEcoO109I74RGCGC/Y

3、HaeII76RG/GWCCYPpuMI77AGT/ACTScaI78AGG/CCTStuI70R/GATCYXhoII73ATAT/TAATAseI79AT/TAATAsnI79AT/CGATBspDI80CC/WGGMvaI100CC/SGGNciI107C/CATGGNcoI108CMG/CKGNspBII90CCANNNN/NTGGPflMI44CCGC/GGSacII104CC/TNAGGSauI96CC/NGGScrFI109CCC/GGGSmaI110CCTGCA/GGSse8387I111CCGC/GGSstII104C/CWWGGStyI112CCANNNN/NTGGVan9

4、1I44CCANNNNN/NNNNTGGXcmI50C/CCGGGXmaI113C/CCGGGXmaCI113(1,611)CCAGTBsrI114, 115CG(10,12)CGANNNNNNTGC(12,10)BcgI116, 117CGRY/CGBsiEI118C/GTACGBsiWI119CG/CGBstUI120C/GGCCGEagI121Y/GGCCRCfrI122Y/GGCCREaeI122C/GGCCGEclXI121CGTCTC(1,5)Esp3I123, 124CG/CGFnuDII120CG/CGMvnI120CGAT/CGPvuI125CG/GWCCGRsrII126C

5、R/CCGGYGSgrAI92CG/CGThaI120C/GGCCGXmaIII121CTC/TTAAGAflII127C/TAGBfaI128C/TTAAGBfrI127CTGGAG(16,14)BpmI129, 130C/TNAGDdeI131CTCTTC(1,4)EarI132, 133C/YCGRGAvaI102CTGAAG(16,14)Eco57I134, 135CTGGAG(16,14)GsuI129, 130C/TAGMaeI128C/TCGAGPaeR7I136CTGCA/GPstI137C/TAGRmaI128C/TRYAGSfcI138C/TCGAGXhoI136(14,1

6、6)CTCCAGBpmI139, 140(14,16)CTGCACBsgI141, 142(14,16)CTTCAGEco57I143, 144(14,16)CTCCAGGsuI139, 140GAGACGT/CAatII145GACN/NNGTCAspI146GAANN/NNTTCAsp7008GACNNN/NNGTCAspEI9GAAGAC(2,6)BbsI147, 148GAAGAC(2,6)BpuAI147, 148GATNN/NNATCBsaBI20GAATGC(1,611)BsmI149, 150GA/TCDpnI only if151G-Me/GATCDpnII151GACNNN

7、N/NNGTCDrdI28GACNNN/NNGTCEam1105I9GAG/CTCEcl136II152R/AATTYAcsI53GR/CGYCAcyI153GR/CGYCAhaII153R/AATTYApoI53GWGCW/CAspHI154GRGCY/CBanII155GDGCH/CBmyI156GR/CGYCBsaHI153GDGCH/CBsp1286I156G/AATTCEcoRI157GAT/ATCEcoRV158GACGC(5,10)HgaI159, 160,GWGCW/CHgiAI154G/ANTCHinfI161GATNN/NNATCMamI20/GATCMboI151GAAG

8、A(8,7)MboII162, 163/GATCNdeII151GDGCH/CNspII156GAGTC(4,5)PleI164, 165GAGCT/CSacI166/GATCSau3AI151GAGCT/CSstI166G/AWTCTfiI167GACN/NNGTCTth111I146GAANN/NNTTCXmnI8(9,5)GATGCSfaNI168, 169(5,4)GATCCAlwI170, 171(5,1)GAGACCBsaI172, 173(5,1)GAGACBsmAI174, 175(4,1)GAAGAGEarI176, 177(5,1)GAGACGEsp3I178, 179(6

9、,7)GAGGMnlI180, 181(5,4)GACTCPleI182, 183GCGCAGC(8,12)BbvI184, 185GCCNNNN/NGGCBglI15GC/TNAGCBpu1102I186G/CGCGCBsePI187G/CGCGCBssHII187GC/TNAGCCelII186GCG/CCfoI188R/CCGGYBsrFI64R/CCGGYCfr10I64GC/TNAGCEspI186GC/NGCFnu4HI189GCG/CHhaI188G/CGCHinPI188GC/NGCItaI189GCC/GGCNaeI190G/CCGGCNgoMI191G/CTAGCNheI1

10、92GC/GGCCGCNotI193RCATG/YNspI67GCATC(5,9)SfaNI194, 195GCATG/CSphI196GCCC/GGGCSrfI197G/CGGAciI198(12,8)GCTGCBbvI199, 200(10,12)GCANNNNNNTCG(12,10)BcgI201, 202(1,1)GCATTCBsmI203, 204(8,4)GCAGGTBspMI205, 206(10,5)GCGTCHgaI207, 208GGG/GTACCAcc65I209G/GWCCAflI210GGATC(4,5)AlwI211, 212GGGCC/CApaI213GG/CGC

11、GCCAscI214G/GTACCAsp718209G/GWCCAvaII210G/GATCCBamHI215G/GYRCCBanI216GGTCTC(1,5)BsaI217, 218G/GTNACCBstEII219R/GATCYBstYI73GR/CGYCAcyI153GR/CGYCAhaII153GRGCY/CBanII155GDGCH/CBmyI156GR/CGYCBsaHI153GDGCH/CBsp1286I156RG/GNCCYDraII74RG/GNCCYEcoO109I74GGATG(9,13)FokI220, 221GGCCGG/CCFseI222RGCGC/YHaeII76

12、GG/CCHaeIII223GGTGA(8,7)HphI224, 225G/GCGCCKasI226GGTAC/CKpnI227GG/CGCCNarI228GGN/NCCNlaIV229GDGCH/CNspII156RG/GWCCYPpuMI77G/GNCCSau96I230GGCCNNNN/NGGCCSfiI49R/GATCYXhoII73GTGT/MKACAccI231G/TGCACAlw44I232G/TGCACApaLI232GTGCAG(16,14)BsgI233, 234GTCTC(1,5)BsmAI235, 236GTA/TACBst1107I237GWGCW/CAspHI154

13、GDGCH/CBmyI156GDGCH/CBsp1286I156GWGCW/CHgiAI154GTY/RACHincII238GTY/RACHindII238GTT/AACHpaI239/GTNACMaeIII240GDGCH/CNspII156GTTT/AAACPmeI241GT/ACRsaI242G/TCGACSalI243G/TGCACSnoI232(6,2)GTCTTCBbsI244, 245(6,2)GTCTTCBpuAI244, 245TAYAC/GTRBsaAI86TAC/GTASnaBI246TCT/CCGGAAccII247T/CCGGABseAI247T/CCGGABspE

14、I247T/CATGABspHI248T/CCGGAMroI247TCG/CGANruI249T/CATGARcaI248T/CGATaqI250T/CTAGAXbaI251(7,8)TCACCHphI252, 253(7,8)TCTTCMboII254, 255TGTGC/GCAAosI256TGC/GCAAviII256TGG/CCABalI257T/GATCABclI258T/GTACABsp1407I259Y/GGCCRCfrI122Y/GGCCREaeI122TGC/GCAFspI256TGG/CCAMluNI257TGG/CCAMscI257TGC/GCAMstI256T/GTAC

15、ASspBI259TTTTT/AAAAhaIII260TT/CGAAAsuII261TT/CGAABstBI261TTT/AAADraI260T/TAAMseI262TT/CGAANspV261TTAAT/TAAPacI263TT/CGAASfuI261T/TAATru9I262去掉切口位置的酶切位点Sequence cutEnzyme NameSEQ ID NO:AARAATTYAcsI53RAATTYApoI53AAGCTTHindIII54AACGTTPsp140 6I55AATATTSspI56ACACRYGTAflIII57ACCGGTAgeI58ACATGTBspLU11I59AC

16、CTGC(4,8)BspMI60, 61ACTGG(1,611)BsrI62, 63RCCGGYBsrFI64RCCGGYCfr10I64ACGTMaeII65ACGCGTMluI66RCATGYNspI67ACCGGTPinAI58ACCWGGTSexAI68ACTAGTSpeI69AGAGGCCTAatI70AGCTAluI71AGATCTBglII72RGATCYBstYI73RGGNCCYDraII74AGCGCTEco47III75RGGNCCYEcoO109I74RGCGCYHaeII76RGGWCCYPpuMI77AGTACTScaI78AGGCCTStuI70RGATCYXho

17、II73ATATTAATAseI79ATTAATAsnI79ATCGATBspDI80ATCGATClaI80ATGCATNsiI81ATTTAAATSwaI82CACAGNNNCTGAlwNI83CACGTGBbrPI84CACNNNGTGDraIII85YACGTRBsaAI86CAATTGMfeI87CAATTGMunI87CATATGNdeI88CATGNlaIII89CMGCKGNspBII90CACGTGPmaCI84CACGTGPmlI84CAGCTGPvuII91CRCCGGYGSgrAI92(13,9)CATCCFokI93, 94CCCCGCAciI95CCTNAGGAoc

18、I96CCWGGApyI97CCTAGGAvrII98CCTAGGBinI98CCNNGGBsaJI99CCNNNNNNNGGBsiYI23CCNNNNNNNGGBslI23CCANNNNNNTGGBssGI27CCWGGBstNI100CCANNNNNNTGGBstXI27CCTNAGGBsu36I96CCRYGGDsaI101CYCGRGAvaI102CCTNNNNNAGGEcoNI32CCWGGEcoRII97CCGGHpaII103CCGCGGKspI104CCTC(7,6)MnlI105, 106CCGGMspI103CCTNAGGMstII96CCWGGMvaI100CCSGGNc

19、iI107CCATGGNcoI108CMGCKGNspBII90CCANNNNNTGGPflMI44CCGCGGSacII104CCTNAGGSauI96CCNGGScrFI109CCCGGGSmaI110CCTGCAGGSse8387I111CCGCGGSstII104CCWWGGStyI112CCANNNNNTGGVan91I44CCANNNNNNNNNTGGXcmI50CCCGGGXmaI113CCCGGGXmaCI113(1,611)CCAGTBsrI114, 115CG(10,12)CGANNNNNNTGC(12,10)BcgI116, 117CGRYCGBsiEI118CGTACG

20、BsiWI119CGCGBstUI120CGGCCGEagI121YGGCCRCfrI122YGGCCREaeI122CGGCCGEclXI121CGTCTC(1,5)Esp3I123, 124CGCGFnuDII120CGCGMvnI120CGATCGPvuI125CGGWCCGRsrII126CRCCGGYGSgrAI92CGCGThaI120CGGCCGXmaIII121CTCTTAAGAflII127CTAGBfaI128CTTAAGBfrI127CTGGAG(16,14)BpmI129, 130CTNAGDdeI131CTCTTC(1,4)EarI132, 133CYCGRGAvaI

21、102CTGAAG(16,14)Eco57I134, 135CTGGAG(16,14)GsuI129, 130CTAGMaeI128CTCGAGPaeR7I136CTGCAGPstI137CTAGRmaI128CTRYAGSfcI138CTCGAGXhoI136(14,16)CTCCAGBpmI139, 140(14,16)CTGCACBsgI141, 142(14,16)CTTCAGEco57I143, 144(14,16)CTCCAGGsuI139, 140GAGACGTCAatII145GACNNNGTCAspI146GAANNNNTTCAsp7008GACNNNNNGTCAspEI9G

22、AAGAC(2,6)BbsI147, 148GAAGAC(2,6)BpuAI147, 148GATNNNNATCBsaBI20GAATGC(1,611)BsmI149, 150GATCDpnI only if151G-MeGATCDpnII151GACNNNNNNGTCDrdI28GACNNNNNGTCEam1105I9GAGCTCEcl136II152RAATTYAcsI53GRCGYCAcyI153GRCGYCAhaII153RAATTYApoI53GWGCWCAspHI154GRGCYCBanII155GDGCHCBmyI156GRCGYCBsaHI153GDGCHCBsp1286I156GAATTCEcoRI157GATATCEcoRV158GACGC(5,10)HgaI159, 160,GWGCWCHgiAI154GANTCHinfI161GATNNNNATCMamI20GATCMboI151GAAGA(8,7)MboII162, 163GATCNdeII151GDGCHCNspII156GAGTC(4,5)PleI164, 165GAGCTCSacI166GATCSau3AI151GAGCTCSstI166GAWTC