AATCC-100-2004.docx

AATCC-100-2004.docx

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AntibacterialFinishesonTextileMaterials:

Assessmentof

Developedin1961byAATCCCommitteeRA31;revised1965,1981,1988（withtitlechange）,1993,1999;editoriallyrevised1969,1971,1974,1985;reaffirmed1977,1981,1989,1998;editoriallyrevisedandreaffirmed1986,2004.

1.PurposeandScope

1.1Thistestmethodprovidesaquantitativeprocedurefortheevaluationofthedegreeofantibacterialactivity.Assessmentofantibacterialfinishesontextilematerialsisdeterminedbythedegreeofantibacterialactivityintendedintheuseofsuchmaterials.Ifonlybacteriostaticactivity（inhibitionofmultiplication）isintended,aqualitativeprocedurewhichclearlydemonstratesantibacterialactivityascontrastedwithlackofsuchactivitybyanuntreatedspecimenmaybeacceptable.However,ifbactericidalactivityisintendedorimplied,quantitativeevaluationisnecessary.Quantitativeevaluationalsoprovidesaclearerpictureforpossibleusesofsuchtreatedtextilematerials.

2.Principle

2.1SwatchesoftestandcontroltextilematerialsaretestedqualitativelyforantibacterialactivitybyAATCCMethod147.Thoseshowingactivityareevaluatedquantitatively.Testandcontrolswatchesareinoculatedwiththetestorganisms.Afterincubation,thebacteriaareelutedfromtheswatchesbyshakinginknownamountsofneutralizingsolution.Thenumberofbacteriapresentinthisliquidisdetermined,andthepercentagereductionbythetreatedspecimeniscalculated.

3.Terminology

3.1activity,n.—ofanantibacterialagent,ameasureofeffectivenessoftheagent.

3.2antibacterialagent,n.一intextiles,anychemicalwhichkillsbacteria（bactericide）orinterfereswiththemultiplication,growthoractivityofbacteria（bacteriostat）.

4.SafetyPrecautions

NOTE:

Thesesafetyprecautionsareforinformationpurposesonly.Theprecautionsareancillarytothetestingproceduresandarenotintendedtobeallinclusive.Itistheuser'sresponsibilitytousesafeandpropertechniquesinhandlingmaterialsinthistestmethod.ManufacturersMUSTbeconsultedforspecificdetailssuchasmaterialsafetydatasheetsandothermanufacturer'srecommendations.AllOSHAstandardsandrulesmustalsobeconsultedandfollowed.

4.1Boththequalitativeandquantitativetestsshouldbecarriedoutbypersonswithtrainingandexperienceintheuseofbacteriologicaltechniques.TheU.S.DepartmentofHealthandHumanServicespublication,BiosafetyinMicrobiologicalandBiomedicalLaboratories,shouldbeconsulted（see13.1）.

4.2CAUTION:

Someofthebacteriausedinthistestarecapableofinfectinghumansandproducingdisease.Therefore,everynecessaryandreasonableprecautionmustbetakentoeliminatethisrisktothelaboratorypersonnelandtopersonnelintheassociatedenvironment.Wearprotectiveclothingandrespiratoryprotectionthatpreventspenetrationbythebacteria.

4.3Goodlaboratorypracticesshouldbefollowed.Wearsafetyglassesinalllaboratoryareas.

4.4Allchemicalsshouldbehandledwithcare.

4.5Aneyewash/safetyshowershouldbelocatednearbyforemergencyuse.

4.6Sterilizeallcontaminatedsamplesandtestmaterialspriortodisposal.

4.7Exposuretochemicalsusedinthisproceduremustbecontrolledatorbelowlevelssetbygovernmentauthorities（e.g.,OccupationalSafetyandHealthAdministration's[OSHA]permissibleexposurelimits[PEL]asfoundin29CFR1910.1000ofJanuary1,1989）.Inaddition,theAmericanConferenceofGovernmentalIndustrialHygienists（ACGIH）ThresholdLimitValues（TLVs）comprisedoftimeweightedaverages（TUATWA）,shorttermexposurelimits（TLV-STEL）andceilinglimits（TLV-C）arerecommendedasageneralguideforaircontaminantexposurewhichshouldbemet（see13.2）.

5.Limitations

5.1Foraqualitative,relativelyquickandeasilyexecutedmethodtodetermineresidualantibacterialactivityoftextilematerials,refertoAATCCMethod147,AntibacterialActivityAssessmentofTextileMaterials:

ParallelStreakMethod.

6.TestOrganisms

6.1Testbacteria.

6.1.1Staphylococcusaureus,AmericanTypeCultureCollectionNo.6538.Grampositiveorganism（see13.10）.

6.1.2Klebsiellapneumoniae,AmericanTypeCultureCollectionNo.4352.Gramnegativeorganism（see13.10）.

6.1.3Othersuitablespeciescanalsobeused.

7.CultureMedium

7.1Suitablebroth/agarmediaareNutrient,TrypticaseSoyandBrain-HeartInfusion.

NutrientBroth:

Peptone（Bacto-peptone）

（see13.3） 5g

Beefextract（see13.4） 3g

Distilledwater to1000mL

7.2Heattoaboiltodisperseingredients.AdjusttopH6.8土0.1withINsodiumhydroxide（NaOH）solution.（Thisisnotnecessaryifprepared,dehydratedmediumisused.）

7.3Dispensein10mLamountsinconventionalbacteriologicalculturetubes（i.e.,125x17mm）.Plugandsterilizeat103kPa（15psi）for15min.

7.4Nutrientagar.Ad