英文生物论文写作AbstractExample01Word下载.docx
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Frist,Whenwritingabstractsofacademicpapers,manyauthorsareusedtoapplyingpasttense;
Ratherthanusingpasttenseaimlessly,theyareadapttouseittodescribethemethodsorresultsorboth(5papers).Whenwritingabstractsofreviews,fewauthorsusepasttense(4reviewsinall,Nopasttense).
Second,Therearethreepapersusingpassivevoiceinalmostwholeabstracts,excepttheseauthorswhoareadeptatdoingthis,othersareprefertoapplypositivevoice.
Third,Academicpapershavedisparatewritingformcomparedwithreviews.Theymustcontainbackgrounds,questions,methods,resultsandconclusions,althoughsometimesmethodsandresultscanbeincorporatedpart.Whilereviewsseemnottohaveafixedpatternofwriting.
Fourth,Onlydoesaspecificabbreviationappearmorethantwiceinanabstract,theuseofthisabbreviationcanberightandstandard.
FinallyIrealizedthatexceptforconciseness,anabstractmustbewell-organizedinordertocatchingtheeyesofreaders.Asanewbieabstractwriters,weneedtolearnalotmore.
Throughreadinganddissectingthese10abstractIhavequestionsasfollows:
1.Whetherweshouldtrytoavoidusinglongandcomplexsentences?
Compoundsentencessometimesseemtoberedundantandbeeasiertomisleadreaders.
2.Insomesituations,forexample“wereportthefirstcloningandfunctionalcharacterizationofanSRmoleculefromteleostfish(Tetraodonnigroviridis).ThisSR(TnSR)……,”Isitnecessarytospecify“TnSR”inadvanceinfirstsentence,thenuseitsabbreviationdirectlyinthetextthatfollows?
Ifitisnecessary,howshouldwedo?
3.Whenwritinganabstract,shouldweusesentenceslike“wecharacterizesforthefirsttime……”,“wereportthefirstcloningandfunctionalcharacterizationof……”,and“toourknowledge……”ect.?
Why?
4.Howshouldwedotostressthenoveltyorsignificanceofourresearch?
5.Whymanyauthorswritesentenceslike“dataontheiroccurrenceandfunctions……arelimited.”or“Todateveryfewstudieshavebeencarriedoutto……”andsoon?
Ifthisusageiscorrect,what’sthefunctionorpurpose?
6.What’sthedifferencebetween“novel(morespecific)”and“new”?
7.Likeacademicpapers,doabstractsofreviewshaveaspecificandstandardwritingform?
8.ByhearingprofessorYu’sexplanation,Iknowninthefollowingexamplewhat“which”referstoisnotveryclear.Istillwonderhowtorewritethissentencecorrectly?
(Example:
However,vitaminAdeprivationparadoxicallyresultedindramaticexpansionofinterleukin-13(IL-13)–producingILC2sandresistancetonematodeinfectioninmice,whichrevealedthatILCsareprimarysensorsofdietarystress.)
2.
Abstract
备注:
蓝色——缩写
黄色——一般现在时
红色——过去时
绿色——被动语态(批注框中的绿色代表修改后的正确形式)
1.
Cheng,S.F.,etal.(2012)."
Asingleimmunoglobulin-domainIgSFproteinfromSciaenopsocellatusregulatespathogen-inducedimmuneresponseinanegativemanner."
DevCompImmunol38
(1):
117-127.
Conclusion
Method4
Method1
Method2
Results
Method3
Background
Theimmunoglobulinsuperfamily(IgSF)isalargegroupofcellsurfaceproteinsthatincludevariousimmunoregulatoryreceptorssuchasnovelimmunetypereceptors(NITRs),whichareafamilyofdiversifiedproteinsfoundexclusivelyinbonyfish.Inthisstudy,weidentifiedandanalyzedanIgSFprotein,SoIgSF1,fromreddrum(Sciaenopsocellatus).SoIgSF1iscomposedof225aminoacidresiduesandmoderatelyrelatedtoteleostNITRs.InsilicoanalysisindicatedthatSoIgSF1isatypeItransmembraneglycoproteinandcontainsanN-terminalsignalpeptidesequence,asingleextracellularimmunoglobulinVdomain,atransmembraneregion,andacytoplasmicregion.However,unlikemostNITRs,thecytoplasmicregionofSoIgSF1exhibitsnoconsensusinhibitoryorstimulatorysignalingsequencesbuthastwotyrosine-containingmotifsthatconformtotheright-halfsequenceoftheimmunoreceptortyrosine-basedinhibitorymotif(ITIM).QuantitativerealtimeRT-PCRanalysisshowedthatSoIgSF1expressionoccurredmainlyinimmuneorgansandwasdrasticallyinducedbyviralandbacterialinfection.Immunofluorescencemicroscopyindicatedthatviralinfectionofheadkidney(HK)leukocytesinducedsurfaceexpressionofSoIgSF1,whichwasabletointeractwithantibodiesagainstrecombinantSoIgSF1.Antibodycross-linkingofSoIgSF1onHKleukocytesinhibitedtheexpressionofimmunerelevantgenesandpromotedviralandbacterialinfection.Takentogether,theseresultsindicatethatSoIgSF1,thoughlackingcanonicalintracellularsignalingmotifs,isinvolvedinregulationofhostimmuneresponseduringpathogeninfectionpossiblybyfunctioningasanegativesignalingreceptorthroughanovelmechanism.
2.Meng,Z.,etal.(2012)."
ScavengerreceptorinfishisalipopolysacchariderecognitionmoleculeinvolvedinnegativeregulationofNF-kappaBactivationbycompetingwithTNFreceptor-associatedfactor2recruitmentintotheTNF-alphasignalingpathway."
JImmunol189(8):
4024-4039.
Backgroundandproblems
Conlusion
Method3
Results
Scavengerreceptors(SRs)playcrucialrolesininnateimmunitybyactingaspatternrecognitionreceptors.AlthoughSRsarewidelydocumentedinmammals,dataontheiroccurrenceandfunctionsinancientvertebratesarelimited.Inthisstudy,wereport,toourknowledge,thefirstcloningandfunctionalcharacterizationofanSRmoleculefromteleostfish(Tetraodonnigroviridis).ThisSR(TnSR)wasidentifiedasahomologtomammalianscavengerreceptorclassAmember5withtheconservedstructureofaclassASR.TnSRcontainedmultidomainsinatypeIItransmembranereceptor,includinganSRcysteine-richdomain,twocoiled-coilcollagenousdomains,atransmmebranedomain,andashortN-terminalintracellularregionwithanunexpectedTNFR-associatedfactor2-bindingconsensusmotifsimilartothatinhumanMSRmolecules.PhylogeneticanalysissuggestedthatTnSRmaybeanancientmemberofclassASRsresultingfromthecloserelationshipbetweenscavengerreceptorclassAmember5andmacrophageSRinvertebratesassociatedwiththesubtledifferencesinTnSRstructure.SubcellularlocalizationanalysisshowedthatTnSRwasacellmembranereceptorwithhomotrimerformsinvolvedintherecognitionandinternalizationofLPSfromsurfacemembranesintolysosomes.Functionally,TnSRexpressionwasdramaticallyinducedbyLPSstimulation.TnSRservedasanegativeregulatorinLPS-inducedNF-kappaBactivationbythecompetitiverecruitmentofTNFR-associatedfactor2fromtheTNF-alphasignalingpathway.Toourknowledge,thisisthefirstreportshowingthatSRplaysaninhibitoryroleinLPS-elicitedinflammationbycross-talkingwiththeTNF-alphainflammatorypathway.ThesefindingscontributetoabetterunderstandingofthebiologicalandevolutionaryhistoryoftheSRfamily.
3.Sun,J.S.,etal.(2012)."
Cynoglossussemilaevisthioredoxin:
areductaseandanantioxidantwithimmunostimulatoryproperty."
CellStressChaperones17(4):
445-455.
Problems
Background
Method2
Method1
Thioredoxin(Trx)isasmallredoxproteinexistingubiquitouslyinalllivingorganismsandplaysanimportantroleinmultiplecellularprocesses,includingtranscriptionalregulationandimmuneresponse.TodateveryfewstudieshavebeencarriedouttoexaminethefunctionofpiscineTrx.Inthisstudy,weidentifiedandanalyzedthefunctionofaTrxhomologue,CsTrx1,fromhalf-smoothtonguesole(Cynoglossussemilaevis).ThededucedaminoacidsequenceofCsTrx1iscomposedof107residuesandshares54.1-60.8%overallidentitieswiththeTrxofotherteleosts.CsTrx1containsthehighlyconservedCXXCmotif,whichinmammalsisknowntobetheactivesite,intheformofCQPC.ExpressionofCsTrx1asdeterminedbyquantitativereal-timereversetranscriptasePCRwashighestinliverandupregulatedintime-dependentmannersbybacterialinfectionandbyexposuretoiron,copper,andhydrogenperoxide.PurifiedrecombinantCsTrx1(rCsTrx1)exhibitedinsulindisulfidereductaseactivityandantioxidantactivity,bothwhich,however,werelostwhenthetwocysteineresiduesintheCQPCmotifweremutatedtoserine.FurtheranalysisshowedthatrCsTrx1wasabletostimulatetheproliferationofheadkidneyleukocytes,upregulatetheexpressionofimmunerelevantgenes,andenhancetheresistanceofleukocytesagainstbacterialinfection.Takentogether,theseresultsindicatethatCsTrx1isabiologicallyactivereductaseandanantioxidantthatrequirestheCXXCmotifforactivityandthatCsTrx1possessescytokine-likeimmunoregulatoryproperty.TheseresultssuggestaroleforCsTrx1inprotectingcellsagainstoxidativestresscausedbyoxidantexposureandpathogeninfection.
4.Xiong,R.,etal.(2012)."
CharacterizationofaPIAS4homologuefromzebrafish:
insightsintoitsconservednegativeregulatorymechanismintheTRIF,MAVS,andIFNsignalingpathwaysduringvertebrateevolution."
JImmunol188(6):
2653-2668.
Backgroundandproblems
Result2
Conclusion
Result1
MembersoftheproteininhibitorofactivatedSTAT(PIAS)familyarekeyregulatorsofvarioushumanandmammaliansignalingpathways,butdataontheiroccurrenceandfunctionsinancientvertebratesarelimited.ThisstudycharacterizesforthefirsttimetoourknowledgeaPIAS4homologue(PIAS4a)fromzebrafish.Structurally,thiszebrafishPIAS4a(zfPIAS4a)sharesanumberofconservedfunctionaldomainswithmam