天然产物化学英文版副本汇总Word下载.docx

天然产物化学英文版副本汇总Word下载.docx

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MyoungJinSon,CatherineW.Rico,SeokHyunNam,andMiYoungKang

Abstract:

Theeffectsoforyzanolandferulicacidontheglucosemetabolismofhigh-fat-fedmicewereinvestigated.MaleC57BL/6Nmicewererandomlydividedinto4groups:

NCgroupfedwithnormalcontroldiet;

HFgroupfedwithhigh-fat（17%）diet;

HF-Ogroupfedwithhigh-fatdietsupplementedwith0.5%oryzanol;

andHF-FAgroupfedwithhigh-fatdietsupplementedwith0.5%ferulicacid.Allanimalswereallowedfreeaccesstotheexperimentaldietsandwaterfor7wk.Attheendoftheexperimentalperiod,theHF-OandHF-FAgroupsexhibitedsignificantlylowerbloodglucoselevelandglucose-6-phosphatase（G6pase）andphosphoenolpyruvatecarboxykinase（PEPCK）activities,andhigherglycogenandinsulinconcentrationsandglucokinase（GK）activitycomparedwithNCandHFgroups.Theresultsofthisstudyillustratethatbothoryzanolandferulicacidcouldreducetheriskofhigh-fatdiet-inducedhyperglycemiaviaregulationofinsulinsecretionandhepaticglucose-regulatingenzymeactivities.

Keywords:

diabetes,ferulicacid,high-fat-fedmice,hypoglycemiceffect,oryzanol

Introduction

Chronicconsumptionofahigh-fatdiethasbeenassociatedwiththedevelopmentofobesityandtype2diabetesmellitus（Hillandothers1992;

Brayandothers2004）.Scientificstudieshaveshownthatexcessiveintakeofdietaryfatresultsinincreasedbodyweightandpoorglucoseregulation（AlsaifandDuwaihy2004;

Petroandothers2004;

Messierandothers2007）.Diabetesischaracterizedbyhyperglycemiathatresultsinthegenerationoffreeradicalsleadingtooxidativestress（West2000）.Duetochangesinlifestylepatterns,particularlypooreatinghabitandsedentarylifestyle,theincidenceofdiabeteshasrapidlyincreasedinepidemicproportions.Around171millioncasesofdiabetesworldwidewerereportedin2001anditwasprojectedthatby2030,366millionpeoplewillhavediabetes（Wildandothers2004）.Withthisincreasingglobalprevalenceofdiabetes,theneedfortherapeuticmeasuresagainstthediseasehasbecomestrongerandmoreurgent.Awiderangeoforalmedicinesarecurrentlybeingusedfortreatingdiabetes.However,variousadverseeffectsandhighratesofsecondaryfailureshavebeenassociatedwiththeavailableantidiabeticmedicines（Inzucchi2002）.Thus,findingnaturaldrugswithhypoglycemicactivityhasnowbecomethefocusofscientistsandresearchers.

Atpresent,thereisaconsiderablepublicandscientificinterestinutilizingphytochemicalsforthetreatmentandpreventionofvariousdiseases.Naturallyoccurringphenoliccompounds,suchasoryzanolandferulicacid,areknowntohavestrongantioxidantactivities（Wangandothers2002;

Srinivasanandothers2007）.Oryzanolisamixtureofferulicacid（4-hydroxy-3-methoxycinnamicacid）esterswithphytosterols（Lerma-Garciaandothers2009）andprimarilyextractedfromricebran.Ferulicacidiscommonlyfoundinfruitsandvegetables,includingbanana,broccoli,ricebran,andcitrusfruits（ZhaoandMoghadasian2008）.Bothoryzanolandferulicacidpossessseveralphysiologicalproperties,suchasreductionofserumcholesterollevels（Wilsonandothers2007）,inhibitionoftumorpromotion（Yasukawaandothers1998）,andprotectiveactionagainstliverinjury（Choti-markornandUshio2008）.Oxidativestressisregardedasthekeyfactorinthedevelopmentofdiabetesanditsassociatedhealthdisorders.Thehigh-fatdietfedC57BL/6mousemodelhaslongbeenusedbyresearchersininvestigatingthepathophysiologyofimpairedglucosetoleranceandtype2diabetesandforthedevelopmentofnewtreatments（Surwitandothers1988;

Surwitandothers1991;

Schreyerandothers1998;

WinzellandAhren2004）.Sincediabetesisafreeradicalmediateddisease,thestrongantioxidantactivityoforyzanolandferulicacidmaybeusefulinpreventingthedevelopmentofdiabetichyperglycemiaunderahigh-fatdiet.Therearelimitedreportsonthephysiologicalfunctionsofthesephenoliccompoundsinrelationtoglucosemetabolisminanimalmodels.Thus,thisstudywasconductedtoinvestigatetheeffectsofdietaryfeedingoforyzanolandferulicacidontheglucosemetabolisminhigh-fat-fedC57BL/6mice.

1.MaterialsandMethods

1.1Animalsanddiets

Twenty-fourmaleC57BL/6Nmiceof4wkofage,weighing12g,wereobtainedfromOrientInc.（Seoul,Korea）.Theywereindividuallyhousedinstainlesssteelcagesinaroommaintainedat25◦Cwith50%relativehumidityand12/12hlight/darkcycleandfedwithapelletizedchowdietfor2wkafterarrival.Themicewerethenrandomlydividedinto4dietarygroups（n=6）.The1stand2ndgroupswerefedwithanormalandhigh-fat（17%,w/w）diets,respectively,whiletheother2groupsweref